Clostridium botulinum botA/BOTOX Recombinant Protein (N-His) (JN888012)

Name: Clostridium botulinum botA/BOTOX Recombinant Protein (N-His)
Brand: abinScience
SKU: JN888012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	JN888012
Description	Recombinant Clostridium botulinum BotA Protein, N-His (JN888012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P0DPI0
Longueur de protéine	Met1-Glu424
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Clostridium botulinum
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	BOTOX, BoNT/A, Bontoxilysin-A, Botulinum neurotoxin A heavy chain, Botulinum neurotoxin A light chain, Botulinum neurotoxin type A, Botulinum neurotoxin type A1, EC:3.4.24.69, HC, LC, atx, bonT, botA
Contexte	Botulinum neurotoxin type A is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. 1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302 2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132 3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397 4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517 5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155 6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415 7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378 8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274
Note	For research use only