ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Clostridium botulinum
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
BOTOX, BoNT/A, Bontoxilysin-A, Botulinum neurotoxin A heavy chain, Botulinum neurotoxin A light chain, Botulinum neurotoxin type A, Botulinum neurotoxin type A1, EC:3.4.24.69, HC, LC, atx, bonT, botA
Фон
Botulinum neurotoxin type A is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.
1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302
2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132
3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397
4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517
5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155
6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415
7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378
8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274
中文
English
한국어
日本語
Español
Français
Русский
