

| Каталожный номер | JN912012 |
|---|---|
| Описание |
Recombinant E. coli groES Protein, N-His (JN912012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | P0A6F9 |
| Длина белка | Met1-Ala97 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Escherichia coli |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 12.55 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | 10 kDa chaperonin, Chaperonin-10, Co-chaperonin GroES, Cpn10, groES, groS, mopB |
| Фон | Co-chaperonin GroES is a ~10 kDa protein. Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL. 1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860 2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100 3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490 4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386 5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317 6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018 7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391 8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517 9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629 |
| Примечание | For research use only |
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