



| Número de catálogo | VK762022 |
|---|---|
| Descripción |
Recombinant HBV C/Capsid protein Protein, C-His (VK762022) expressed in E. coli, spanning Met1-Val149.
Highlights
|
| Sistema de expresión | E. coli |
| Número de acceso | P03146 |
| Longitud de proteína | Met1-Val149 |
| Aplicaciones | SDS-PAGE, WB, ELISA, Immunogen, Bioactivity testing in progress |
| Especies | Hepatitis B virus (HBV) |
| Naturaleza | Recombinant |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >95% as determined by SDS-PAGE. |
| Peso molecular predicho | 17.92 kDa |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Reconstitución | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Envío | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Nombres alternativos | Capsid protein, C, Core antigen, Core protein, HBcAg, p21.5, Hepatitis B Virus (HBV) |
| Fondo | Capsid protein is a ~21 kDa protein. Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. |
| Nota | For research use only. |

SDS-PAGE for Recombinant HBV C/Capsid protein Protein, C-His

Detected by E21 (Catalog No: VK762023) in indirect ELISA
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