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HBV HBsAg Recombinant Protein (C-S Tag) (VK539011)

HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
HBV HBsAg Recombinant Protein (C-S Tag)
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  • 100ug
  • 1mg
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  • Aperçu

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  • Reférences

  • Fiche technique

  • SDS

Aperçu
Numéro de catalogueVK539011
Description
Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag (VK539011) expressed in Mammalian Cells, spanning Met175-Ile400. Purity: >90% by SDS-PAGE.
Highlights
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionMammalian cells
Numéro d'accèsQ9E6S4
Longueur de protéineMet175-Ile400
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHepatitis B virus (HBV)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 29.41 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsLarge envelope protein, L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen, S
Contexte

Large envelope protein (HBsAg) is a ~43 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.

Note For research use only.
Images
  • HBV HBsAg Recombinant Protein (C-S Tag)

    SDS-PAGE

    SDS-PAGE for Recombinant HBV S/L-HBsAg/L glycoprotein Protein, C-S Tag

  • HBV HBsAg Recombinant Protein (C-S Tag)

    Bioactivity

    Detects HBV HBsAg antibody in indirect ELISAs.

  • HBV HBsAg Recombinant Protein (C-S Tag)

    Bioactivity

    Detects Exbivirumab in indirect ELISAs.

  • HBV HBsAg Recombinant Protein (C-S Tag)

    Bioactivity

    Detects HBV HBsAg antibody in indirect ELISAs.

  • HBV HBsAg Recombinant Protein (C-S Tag)

    Bioactivity

    Detects Libivirumab in indirect ELISAs.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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