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HCV Capsid Recombinant Protein (N-GST & C-His) (VK506032)

HCV Capsid Recombinant Protein (N-GST & C-His)
HCV Capsid Recombinant Protein (N-GST & C-His)
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  • 50ug
  • 100ug
  • 1mg
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  • 概要

  • イメージ

  • 参考文献

  • データシート

  • SDS

概要
カタログ番号VK506032
説明
Recombinant HCV Capsid Protein, N-GST & C-His (VK506032) expressed in E. coli, spanning Asp21-Trp96. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムE. coli
アクセッション番号Q81258
タンパク質長Asp21-Trp96
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Hepatitis C virus (HCV)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 36.64 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名Genome polyprotein, Core protein precursor, Capsid protein C, p23, Mature core protein, p21, Envelope glycoprotein E1, gp32, gp35, Envelope glycoprotein E2, NS1, gp68, gp70, Viroporin p7, Protease NS2, p23, EC:3.4.22.-, Non-structural protein 2, NS2, Serine protease/helicase NS3, EC:3.4.21.98, EC:3.6.1.15, EC:3.6.4.13, Hepacivirin, NS3 helicase, NS3 protease, NS3P, Viroporin p70, Non-structural protein 4A, NS4A, p8, Non-structural protein 4B, NS4B, p27, Non-structural protein 5A, NS5A, p56/58, RNA-directed RNA polymerase, EC:2.7.7.48, NS5B, p68
背景

Genome polyprotein is a ~329 kDa protein. Packages viral RNA to form a viral nucleocapsid, and promotes virion budding (Probable). Participates in the viral particle production as a result of its interaction with the non-structural protein 5A. Binds RNA and may function as a RNA chaperone to induce the RNA structural rearrangements taking place during virus replication. Modulates viral translation initiation by interacting with viral IRES and 40S ribosomal subunit. Affects various cell signaling pathways, host immunity and lipid metabolism (Probable).

1. Jackel-Cram, C. et al. (2007) Journal of hepatology 46, 999-1008. PMID: 17188392
注意事項 For research use only
イメージ
  • HCV Capsid Recombinant Protein (N-GST & C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant HCV Capsid Protein, N-GST & C-His

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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