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HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
HIV-1 gp120 Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK617011
Описание
Recombinant HIV1 GP140 Protein, C-His (VK617011) expressed in Mammalian Cells, spanning Met23-Ile680. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииMammalian cells
Номер доступаQ78225
Длина белкаMet23-Ile680
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHuman immunodeficiency virus type 1 (HIV-1)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 85.10 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEnvelope glycoprotein gp160, Env polyprotein, Surface protein gp120, SU, Glycoprotein 120, gp120, Transmembrane protein gp41, TM, Glycoprotein 41, gp41, env
Фон

Envelope glycoprotein gp160 (gp140/Glycoprotein 140) is a ~97 kDa protein. Envelope glycoprotein gp160: Oligomerizes in the host endoplasmic reticulum into predominantly trimers. In a second time, gp160 transits in the host Golgi, where glycosylation is completed. The precursor is then proteolytically cleaved in the trans-Golgi and thereby activated by cellular furin or furin-like proteases to produce gp120 and gp41.

Примечание For research use only.
Изображения
  • HIV-1 gp120 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant HIV1 GP140 Protein, C-His

  • HIV-1 gp120 Recombinant Protein (C-His)

    Bioactivity

    Detects Zinlirvimab in indirect ELISAs.

  • HIV-1 gp120 Recombinant Protein (C-His)

    Bioactivity

    Detects Elipovimab in indirect ELISAs.

  • HIV-1 gp120 Recombinant Protein (C-His)

    Bioactivity

    Detects Iv0111 in indirect ELISAs.

  • HIV-1 gp120 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Anti-HIV1 gp120/Glycoprotein 120 (VRC01LS) (Catalog No: VK617066) in indirect ELISA.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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