HSV1/HHV-1 ICP0 Recombinant Protein (N-His) (VK083012)

Name: HSV1/HHV-1 ICP0 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK083012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	VK083012
Descripción	Recombinant HSV1/HHV-1 ICP0 Protein, N-His (VK083012) expressed in E. coli, spanning Leu639-Ser762. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P08393
Longitud de proteína	Leu639-Ser762
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Human herpesvirus 1 (HHV-1) (Human herpes simplex virus 1) (HSV-1)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	15.84 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	E3 ubiquitin-protein ligase ICP0, EC:2.3.2.27, Alpha-0 protein, Immediate-early protein IE110, RING-type E3 ubiquitin transferase ICP0, Trans-acting transcriptional protein ICP0, VMW110, ICP0
Fondo	E3 ubiquitin-protein ligase ICP0 is a ~78 kDa protein. SUMO-targeted ubiquitin ligase that plays an essential role in nuclear antiviral defense evasion triggered by dsDNA viruses. Acts during the initial stages of lytic infection and the reactivation of latent viral genome. Prevents the antiviral effect of nuclear bodies by degrading host PML, SP100, MORC2 and MORC3. Prevents antiviral response to viral DNA induced by IFI16 by degrading it. Additionally, inhibits host IRF3 nuclear signaling to prevent interferon production by the infected cells. 1. Hembram, DSS. et al. (2020) Journal of molecular biology 432, 1952-1977. PMID: 32001251 2. Sloan, E. et al. (2016) Journal of virology 90, 8621-33. PMID: 27440897 3. Gaidt, MM. et al. (2021) Nature 600, 138-142. PMID: 34759314 4. Roubille, S. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2412258121. PMID: 39589886
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant HSV1/HHV-1 ICP0 Protein, N-His

Referencias

Datasheet

HSV1/HHV-1 ICP0 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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