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| カタログ番号 | VK083012 |
|---|---|
| 説明 |
Recombinant HSV1/HHV-1 ICP0 Protein, N-His (VK083012) expressed in E. coli, spanning Leu639-Ser762. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P08393 |
| タンパク質長 | Leu639-Ser762 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Human herpesvirus 1 (HHV-1) (Human herpes simplex virus 1) (HSV-1) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.84 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | E3 ubiquitin-protein ligase ICP0, EC:2.3.2.27, Alpha-0 protein, Immediate-early protein IE110, RING-type E3 ubiquitin transferase ICP0, Trans-acting transcriptional protein ICP0, VMW110, ICP0 |
| 背景 | E3 ubiquitin-protein ligase ICP0 is a ~78 kDa protein. SUMO-targeted ubiquitin ligase that plays an essential role in nuclear antiviral defense evasion triggered by dsDNA viruses. Acts during the initial stages of lytic infection and the reactivation of latent viral genome. Prevents the antiviral effect of nuclear bodies by degrading host PML, SP100, MORC2 and MORC3. Prevents antiviral response to viral DNA induced by IFI16 by degrading it. Additionally, inhibits host IRF3 nuclear signaling to prevent interferon production by the infected cells. 1. Hembram, DSS. et al. (2020) Journal of molecular biology 432, 1952-1977. PMID: 32001251 2. Sloan, E. et al. (2016) Journal of virology 90, 8621-33. PMID: 27440897 3. Gaidt, MM. et al. (2021) Nature 600, 138-142. PMID: 34759314 4. Roubille, S. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2412258121. PMID: 39589886 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant HSV1/HHV-1 ICP0 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
