Human ABCD4 Recombinant Protein (N-His) (HT035012)

Name: Human ABCD4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT035012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор
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Обзор

Каталожный номер	HT035012
Описание	Recombinant Human ABCD4 Protein, N-His (HT035012) expressed in E. coli, spanning Leu356-Glu606. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O14678
Длина белка	Leu356-Glu606
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	ATP-binding cassette sub-family D member 4, ABCD4, PXMP1-L, PMP70-related protein, Peroxisomal membrane protein 1-like, P70R, PMP69, Peroxisomal membrane protein 69, PXMP1L, Lysosomal cobalamin transporter ABCD4
Фон	Lysosomal cobalamin transporter ABCD4 is a ~68 kDa protein. Lysosomal membrane protein that transports cobalamin (Vitamin B12) from the lysosomal lumen to the cytosol in an ATP-dependent manner. Targeted by LMBRD1 lysosomal chaperone from the endoplasmic reticulum to the lysosomal membrane. Then forms a complex with lysosomal chaperone LMBRD1 and cytosolic MMACHC to transport cobalamin across the lysosomal membrane. 1. Coelho, D. et al. (2012) Nature genetics 44, 1152-5. PMID: 22922874 2. Fettelschoss, V. et al. (2017) The Journal of biological chemistry 292, 11980-11991. PMID: 28572511 3. Xu, D. et al. (2019) Cell research 29, 1039-1041. PMID: 31467407 4. Kitai, K. et al. (2021) The Journal of biological chemistry 296, 100654. PMID: 33845046 5. Kawaguchi, K. et al. (2016) Scientific reports 6, 30183. PMID: 27456980 6. Deme, JC. et al. (2014) Molecular membrane biology 31, 250-61. PMID: 25535791
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ABCD4 Protein, N-His

Ссылки

Даташит

Human ABCD4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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