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| カタログ番号 | HY429012 |
|---|---|
| 説明 |
Recombinant Human ACADM Protein, N-His (HY429012) expressed in E. coli, spanning Lys26-Asn421. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P11310 |
| タンパク質長 | Lys26-Asn421 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 45.94 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | MCADH, MCAD, ACADM, Medium chain acyl-CoA dehydrogenase, Medium-chain specific acyl-CoA dehydrogenase, mitochondrial |
| 背景 | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) is a ~46 kDa protein. Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase has preference for fatty acyl-CoAs with saturated 6 to 12 carbons long primary chains, making it but can also catalyze longer chains such as C14 and C16. 1. Bross, P. et al. (1990) The Journal of biological chemistry 265, 7116-9. PMID: 1970566 2. He, M. et al. (2011) Molecular genetics and metabolism 102, 418-29. PMID: 21237683 3. Kelly, DP. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 9236-40. PMID: 2251268 4. Nandy, A. et al. (1996) Biochemistry 35, 12402-11. PMID: 8823175 5. Toogood, HS. et al. (2004) The Journal of biological chemistry 279, 32904-12. PMID: 15159392 6. Małecki, J. et al. (2015) The Journal of biological chemistry 290, 423-34. PMID: 25416781 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ACADM Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
