Human ACO1 Recombinant Protein (N-His) (HB808012)

Name: Human ACO1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB808012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Даташит
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Обзор

Каталожный номер	HB808012
Описание	Recombinant Human ACO1 Protein, N-His (HB808012) expressed in E. coli, spanning Lys26-Ile243. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P21399
Длина белка	Lys26-Ile243
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	26.95 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Ferritin repressor protein, IRP1, ACO1, IREB1, Citrate hydro-lyase, IRE-BP 1, Iron-responsive element-binding protein 1, Aconitase, Cytoplasmic aconitate hydratase, Iron regulatory protein 1
Фон	Cytoplasmic aconitate hydratase (ACO1) is a ~98 kDa protein. Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA. 1. Haile, DJ. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 11735-9. PMID: 1281544 2. Kaptain, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 10109-13. PMID: 1946430 3. Philpott, CC. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7321-5. PMID: 8041788 4. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ACO1 Protein, N-His

Ссылки

Даташит

Human ACO1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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