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| カタログ番号 | HX034012 |
|---|---|
| 説明 |
Recombinant Human ACTB/β-actin/Beta Actin Protein, N-His (HX034012) expressed in E. coli, spanning Met1-Phe375. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P60709 |
| タンパク質長 | Met1-Phe375 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Beta-actin, ACTB, Actin, cytoplasmic 1 |
| 背景 | Actin, cytoplasmic 1 (ACTB/β-actin) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Hundt, N. et al. (2014) The FEBS journal 281, 5279-91. PMID: 25255767 _x005F_x005F_x000D_
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2. Drazic, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4399-4404. PMID: 29581253 _x005F_x005F_x000D_
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3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947 _x005F_x005F_x000D_
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4. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809 _x005F_x005F_x000D_
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5. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661 _x005F_x005F_x000D_ |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ACTB/β-actin/Beta Actin Protein, N-His
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