Human ACTB/β-actin/Beta Actin Recombinant Protein (N-His) (HX034012)

Name: Human ACTB/β-actin/Beta Actin Recombinant Protein (N-His)
Brand: abinScience
SKU: HX034012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HX034012
Описание	Recombinant Human ACTB/β-actin/Beta Actin Protein, N-His (HX034012) expressed in E. coli, spanning Met1-Phe375. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P60709
Длина белка	Met1-Phe375
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Beta-actin, ACTB, Actin, cytoplasmic 1
Фон	Actin, cytoplasmic 1 (ACTB/β-actin) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Hundt, N. et al. (2014) The FEBS journal 281, 5279-91. PMID: 25255767 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Drazic, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4399-4404. PMID: 29581253 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661 _x005F_x005F_x000D_
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ACTB/β-actin/Beta Actin Protein, N-His

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Даташит

Human ACTB/β-actin/Beta Actin Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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