Human ADH7 Recombinant Protein (N-His) (HA625012)

Name: Human ADH7 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA625012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HA625012
Description	Recombinant Human ADH7 Protein, N-His (HA625012) expressed in E. coli, spanning Gly17-Phe386. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P40394
Longueur de protéine	Gly17-Phe386
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	41.94 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	All-trans-retinol dehydrogenase [NAD(+)] ADH7; 1.1.1.105; Alcohol dehydrogenase class 4 mu/sigma chain; 1.1.1.1; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Omega-hydroxydecanoate dehydrogenase ADH7; 1.1.1.66; Retinol dehydrogenase; ADH7
Contexte	All-trans-retinol dehydrogenase [NAD(+)] ADH7 is a ~41 kDa protein. Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives. Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid. In vitro can also catalyze the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives. Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal. Catalyzes in the oxidative direction with higher efficiency. 1. Martras, S. et al. (2004) Archives of biochemistry and biophysics 430, 210-7. PMID: 15369820 2. Gallego, O. et al. (2006) The Biochemical journal 399, 101-9. PMID: 16787387 3. Allali-Hassani, A. et al. (1998) FEBS letters 426, 362-6. PMID: 9600267
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human ADH7 Protein, N-His

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Human ADH7 Recombinant Protein (N-His).pdf

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Formula

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