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| カタログ番号 | HW686012 |
|---|---|
| 説明 |
Recombinant Human AHR Protein, N-His (HW686012) expressed in E. coli, spanning Val23-Gln273. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P35869 |
| タンパク質長 | Val23-Gln273 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 30.4 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | bHLHe76, AhR, Ah receptor, AHR, Aryl hydrocarbon receptor, Class E basic helix-loop-helix protein 76, BHLHE76 |
| 背景 | Aryl hydrocarbon receptor (AHR) is a ~96 kDa protein. Ligand-activated transcription factor that enables cells to adapt to changing conditions by sensing compounds from the environment, diet, microbiome and cellular metabolism, and which plays important roles in development, immunity and cancer. Upon ligand binding, translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation. Xenobiotics can act as ligands: upon xenobiotic-binding, activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. 1. MacPherson, L. et al. (2013) Nucleic acids research 41, 1604-21. PMID: 23275542 2. Gomez, A. et al. (2018) The Biochemical journal 475, 3827-3846. PMID: 30373764 3. Sadik, A. et al. (2020) Cell 182, 1252-1270.e34. PMID: 32818467 4. Ema, M. et al. (1994) The Journal of biological chemistry 269, 27337-43. PMID: 7961644 5. Puga, A. et al. (2002) Chemico-biological interactions 141, 117-30. PMID: 12213388 6. Borovok, N. et al. (2020) Frontiers in genetics 11, 582796. PMID: 33193710 7. Haidar, R. et al. (2021) Scientific reports 11, 18194. PMID: 34521881 9. Zhang, X. et al. (2020) Journal of agricultural and food chemistry 68, 10678-10684. PMID: 32866000 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human AHR Protein, N-His
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