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Human AK4/AK3L1 Recombinant Protein (N-His) (HC918012)

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Aperçu
Numéro de catalogueHC918012
Description
Recombinant Human AK4/AK3L1 Protein, N-His (HC918012) expressed in E. coli, spanning Met1-Tyr223. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP27144
Longueur de protéineMet1-Tyr223
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 27.43 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsAK3, AK3L1, AK4, Adenylate kinase 3-like, Adenylate kinase 4, mitochondrial, EC:2.7.4.4, EC:2.7.4.6, GTP:AMP phosphotransferase AK4
Contexte

Adenylate kinase 4, mitochondrial is a ~25 kDa protein. Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions. Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP. Phosphorylates only AMP when using GTP as phosphate donor. In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor. Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors.

1. Liu, R. et al. (2009) Biochemical and biophysical research communications 379, 92-7. PMID: 19073142
2. Panayiotou, C. et al. (2010) The international journal of biochemistry & cell biology 42, 62-9. PMID: 19766732
3. Amiri, M. et al. (2013) The international journal of biochemistry & cell biology 45, 925-31. PMID: 23416111
4. Lanning, NJ. et al. (2014) Cell reports 7, 907-17. PMID: 24767988
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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