ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
91.06 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
AKAP8, A-kinase anchor protein 8, AKAP95, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95
Фон
A-kinase anchor protein 8 (AKAP8) is a ~76 kDa protein. Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis.
1. Eide, T. et al. (1998) Experimental cell research 238, 305-16. PMID: 9473338
2. Collas, P. et al. (1999) The Journal of cell biology 147, 1167-80. PMID: 10601332
3. Steen, RL. et al. (2000) The Journal of cell biology 149, 531-6. PMID: 10791967
4. Eide, T. et al. (2002) EMBO reports 3, 426-32. PMID: 11964380
5. Arsenijevic, T. et al. (2004) The Biochemical journal 378, 673-9. PMID: 14641107
6. Li, Y. et al. (2006) Genes & development 20, 2566-79. PMID: 16980585
7. Gao, X. et al. (2012) Molecular biology of the cell 23, 503-15. PMID: 22130794
8. Eide, T. et al. (2003) The Journal of biological chemistry 278, 26750-6. PMID: 12740381
9. Jiang, H. et al. (2013) Nature structural & molecular biology 20, 1156-63. PMID: 23995757
10. Kamada, S. et al. (2005) Molecular and cellular biology 25, 9469-77. PMID: 16227597
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human AKAP8 Protein, N-GST
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