ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
38.02 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
Aldo-keto reductase family 1 member B1, 1.1.1.300, 1.1.1.372, 1.1.1.54, Aldehyde reductase, Aldose reductase, AR, 1.1.1.21, AKR1B1, ALDR1, ALR2
Fondo
Aldo-keto reductase family 1 member B1 (AKR1B1) is a ~35 kDa protein. Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal.
1. Hamada, Y. et al. (1991) Diabetes 40, 1233-40. PMID: 1936586
2. Crosas, B. et al. (2003) The Biochemical journal 373, 973-9. PMID: 12732097
3. Kabututu, Z. et al. (2009) Journal of biochemistry 145, 161-8. PMID: 19010934
4. Liu, SQ. et al. (1993) Biochimica et biophysica acta 1164, 268-72. PMID: 8343525
5. Spite, M. et al. (2007) The Biochemical journal 405, 95-105. PMID: 17381426
6. Shen, Y. et al. (2011) Chemico-biological interactions 191, 192-8. PMID: 21329684
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human AKR1B1 Protein, N-His
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