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| カタログ番号 | HB610012 |
|---|---|
| 説明 |
Recombinant Human AKT1 Protein, N-His (HB610012) expressed in E. coli, spanning Met1-Ala480. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P31749 |
| タンパク質長 | Met1-Ala480 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 60.57 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB |
| 背景 | RAC-alpha serine/threonine-protein kinase (AKT1) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. 1. Nicholson, KM. et al. (2002) Cellular signalling 14, 381-95. PMID: 11882383 2. Rönnstrand, L. (2004) Cellular and molecular life sciences : CMLS 61, 2535-48. PMID: 15526160 3. Cartlidge, RA. et al. (2005) The EMBO journal 24, 1696-705. PMID: 15861136 4. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781 5. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960 6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173 7. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human AKT1 Protein, N-His
Detected by SAA1231 (Catalog No: HB610033) in indirect ELISA.
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