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| カタログ番号 | HY052012 |
|---|---|
| 説明 |
Recombinant Human ANG Protein, N-His (HY052012) expressed in E. coli, spanning Gln25-Pro147. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P03950 |
| タンパク質長 | Gln25-Pro147 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Ribonuclease 5, Angiogenin, RNase 5, RNASE5, ANG |
| 背景 | Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36. 1. Xu, ZP. et al. (2002) Biochemical and biophysical research communications 294, 287-92. PMID: 12051708 2. Saxena, SK. et al. (1992) The Journal of biological chemistry 267, 21982-6. PMID: 1400510 3. Yamasaki, S. et al. (2009) The Journal of cell biology 185, 35-42. PMID: 19332886 4. Emara, MM. et al. (2010) The Journal of biological chemistry 285, 10959-68. PMID: 20129916 5. Ivanov, P. et al. (2011) Molecular cell 43, 613-23. PMID: 21855800 6. Thiyagarajan, N. et al. (2012) Nature communications 3, 1121. PMID: 23047679 7. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625 8. Shapiro, R. et al. (1986) Biochemistry 25, 3527-32. PMID: 2424496 9. Harper, JW. et al. (1988) Proceedings of the National Academy of Sciences of the United States of America 85, 7139-43. PMID: 2459697 10. Lee, FS. et al. (1989) Biochemical and biophysical research communications 161, 121-6. PMID: 2730651 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ANG Protein, N-His
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