Human APOBEC3D Recombinant Protein (N-His) (HA397012)

Name: Human APOBEC3D Recombinant Protein (N-His)
Brand: abinScience
SKU: HA397012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HA397012
Описание	Recombinant Human APOBEC3D Protein, N-His (HA397012) expressed in E. coli, spanning Met1-Gln386. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q96AK3
Длина белка	Met1-Gln386
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Характер	Recombinant
Цель	ABC3D
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	48.76 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from PBS pH7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	DNA dC->dU-editing enzyme APOBEC-3D, A3D, A3DE, EC:3.5.4.38, APOBEC3D
Фон	DNA dC->dU-editing enzyme APOBEC-3D (ABC3D) is a ~46 kDa protein. DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. 1. Dang, Y. et al. (2006) Journal of virology 80, 10522-33. PMID: 16920826 2. Stenglein, MD. et al. (2010) Nature structural & molecular biology 17, 222-9. PMID: 20062055 3. Hultquist, JF. et al. (2011) Journal of virology 85, 11220-34. PMID: 21835787 4. Liang, W. et al. (2016) PloS one 11, e0157220. PMID: 27428332
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human APOBEC3D Protein, N-His

Ссылки

Даташит

Human APOBEC3D Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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