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| カタログ番号 | HF739012 |
|---|---|
| 説明 |
Recombinant Human APOE Protein, N-His (HF739012) expressed in E. coli, spanning Lys19-His317. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P02649 |
| タンパク質長 | Lys19-His317 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.53 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Apo-E, Apolipoprotein E, APOE |
| 背景 | Apolipoprotein E (APOE) is a ~36 kDa protein. APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. 1. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908 2. Arnon, R. et al. (1991) Biochimica et biophysica acta 1085, 336-42. PMID: 1911868 3. Marcel, YL. et al. (1983) Biochimica et biophysica acta 750, 411-7. PMID: 6860692 4. Sehayek, E. et al. (1991) The Journal of biological chemistry 266, 18259-67. PMID: 1917954 5. Verghese, PB. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1807-16. PMID: 23620513 6. Kowal, RC. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5810-4. PMID: 2762297 7. Ji, ZS. et al. (1997) The Journal of biological chemistry 272, 31285-92. PMID: 9395455 8. Li, X. et al. (2003) Biochemistry 42, 10406-17. PMID: 12950167 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human APOE Protein, N-His
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