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Human AQP2 Recombinant Protein (N-GST) (HW645012)

Human AQP2 Recombinant Protein (N-GST)
Human AQP2 Recombinant Protein (N-GST)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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Vista general
Número de catálogoHW645012
Descripción
Recombinant Human AQP2 Protein, N-GST (HW645012) expressed in E. coli, spanning His177-Ala271. Purity: >90% by SDS-PAGE.
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP41181
Longitud de proteínaHis177-Ala271
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 37.45 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosAquaporin-2, AQP-CD, AQP2, Water channel protein for renal collecting duct, AQP-2, WCH-CD, Collecting duct water channel protein, Aquaporin-CD, ADH water channel
Fondo

Aquaporin-2 (AQP2) is a ~28 kDa protein. Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol.

1. de Mattia, F. et al. (2004) Human molecular genetics 13, 3045-56. PMID: 15509592
2. Sasaki, S. et al. (1994) The Journal of clinical investigation 93, 1250-6. PMID: 7510718
3. van Lieburg, AF. et al. (1994) American journal of human genetics 55, 648-52. PMID: 7524315
4. Deen, PM. et al. (1994) Science (New York, N.Y.) 264, 92-5. PMID: 8140421
5. Abrami, L. et al. (1996) Pflugers Archiv : European journal of physiology 431, 408-14. PMID: 8584435
Nota For research use only.
Imágenes
  • Human AQP2 Recombinant Protein (N-GST)

    SDS-PAGE

    SDS-PAGE for Recombinant Human AQP2 Protein, N-GST

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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