Human ARHGEF15 Recombinant Protein (N-His) (HB586012)

Name: Human ARHGEF15 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB586012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HB586012
Descripción	Recombinant Human ARHGEF15 Protein, N-His (HB586012) expressed in E. coli, spanning Arg619-Thr741. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O94989
Longitud de proteína	Arg619-Thr741
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	16.24 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF; ARHGEF15; KIAA0915
Fondo	Rho guanine nucleotide exchange factor 15 is a ~91 kDa protein. Guanine nucleotide exchange factor (GEF) that activates RhoA, playing a role in the regulation of actin cytoskeleton organization. Is involved in the regulation of vascular smooth muscle contractility. Additionally, it negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2. Does not activate RAC1 or CDC42. 1. Ding, X. et al. (2023) Acta neuropathologica 145, 681-705. PMID: 36929019 2. Ogita, H. et al. (2003) Circulation research 93, 23-31. PMID: 12775584
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human ARHGEF15 Protein, N-His

Referencias

Datasheet

Human ARHGEF15 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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