Human ATF4 Recombinant Protein (N-His) (HB073012)

Name: Human ATF4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB073012
Price: 211 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB073012
説明	Recombinant Human ATF4 Protein, N-His (HB073012) expressed in E. coli, spanning Met1-Pro351. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	P18848
タンパク質長	Met1-Pro351
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	40.75 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	TaxREB67, ATF4, cAMP-dependent transcription factor ATF-4, TXREB, CREB2, cAMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, Activating transcription factor 4, CREB-2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2
背景	Cyclic AMP-dependent transcription factor ATF-4 (ATF4/CREB2) is a ~38 kDa protein. Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation. 1. Sayer, JA. et al. (2006) Nature genetics 38, 674-81. PMID: 16682973 2. Köditz, J. et al. (2007) Blood 110, 3610-7. PMID: 17684156 3. Balsa, E. et al. (2019) Molecular cell 74, 877-890.e6. PMID: 31023583 4. Wang, X. et al. (2021) Molecular psychiatry 26, 1346-1360. PMID: 31444471 5. Guo, X. et al. (2020) Nature 579, 427-432. PMID: 32132707 6. Fessler, E. et al. (2020) Nature 579, 433-437. PMID: 32132706 9. Su, N. et al. (2008) The Journal of biological chemistry 283, 35106-17. PMID: 18940792 10. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988
注意事項	For research use only.