ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
23.25 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Cyclic AMP-dependent transcription factor ATF-5 (ATF5) is a ~30 kDa protein. Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate.
1. Pati, D. et al. (1999) Molecular and cellular biology 19, 5001-13. PMID: 10373550
2. Liu, W. et al. (2004) Biochemical and biophysical research communications 321, 954-60. PMID: 15358120
3. Yamazaki, T. et al. (2010) Life sciences 87, 294-301. PMID: 20654631
4. Dluzen, D. et al. (2011) The Journal of biological chemistry 286, 7705-13. PMID: 21212266
5. Leong, DT. et al. (2012) Journal of cellular biochemistry 113, 2744-53. PMID: 22442021
6. Liu, DX. et al. (2011) Molecular and cellular biology 31, 3906-16. PMID: 21791614
7. Pascual, M. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 1063-72. PMID: 18332083
8. Gho, JW. et al. (2008) Cancer research 68, 6743-51. PMID: 18701499
9. Liu, X. et al. (2012) The Journal of biological chemistry 287, 19599-609. PMID: 22528486
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Human ATF5 Recombinant Protein (N-His)
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