Human ATG14 Recombinant Protein (N-His) (HP189012)

Name: Human ATG14 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP189012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HP189012
Описание	Recombinant Human ATG14 Protein, N-His (HP189012) expressed in E. coli, spanning Glu209-Glu415. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q6ZNE5
Длина белка	Glu209-Glu415
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	25.24 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	ATG14L, ATG14, Barkor, Atg14L, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, KIAA0831
Фон	Beclin 1-associated autophagy-related key regulator (ATG14) is a ~55 kDa protein. Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. 1. Itakura, E. et al. (2008) Molecular biology of the cell 19, 5360-72. PMID: 18843052 2. Sun, Q. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 19211-6. PMID: 19050071 3. Matsunaga, K. et al. (2009) Nature cell biology 11, 385-96. PMID: 19270696 4. Matsunaga, K. et al. (2010) The Journal of cell biology 190, 511-21. PMID: 20713597 5. Fogel, AI. et al. (2013) Molecular and cellular biology 33, 3675-88. PMID: 23878393 6. Diao, J. et al. (2015) Nature 520, 563-6. PMID: 25686604 7. Shi, X. et al. (2023) Cell reports 42, 113045. PMID: 37632749
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ATG14 Protein, N-His

Ссылки

Даташит

Human ATG14 Recombinant Protein (N-His).pdf

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Formula

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