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| カタログ番号 | HV479012 |
|---|---|
| 説明 |
Recombinant Human ATG16L1 Protein, N-GST (HV479012) expressed in E. coli, spanning Met85-Gly284. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q676U5 |
| タンパク質長 | Met85-Gly284 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 49.79 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ATG16L1, Autophagy-related protein 16-1, APG16L, APG16-like 1 |
| 背景 | Autophagy-related protein 16-1 (ATG16L1) is a ~68 kDa protein. Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling. During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8. Thereby, controls the elongation of the nascent autophagosomal membrane. As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. 1. Boada-Romero, E. et al. (2013) The EMBO journal 32, 566-82. PMID: 23376921 2. Nishimura, T. et al. (2013) EMBO reports 14, 284-91. PMID: 23392225 3. Murthy, A. et al. (2014) Nature 506, 456-62. PMID: 24553140 4. Dooley, HC. et al. (2014) Molecular cell 55, 238-52. PMID: 24954904 5. Boada-Romero, E. et al. (2016) Nature communications 7, 11821. PMID: 27273576 6. Fletcher, K. et al. (2018) The EMBO journal 37. PMID: 29317426 7. Lystad, AH. et al. (2019) Nature cell biology 21, 372-383. PMID: 30778222 8. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989 9. Lei, Y. et al. (2012) Immunity 36, 933-46. PMID: 22749352 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ATG16L1 Protein, N-GST
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