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| カタログ番号 | HP478012 |
|---|---|
| 説明 |
Recombinant Human ATG4A Protein, N-His (HP478012) expressed in E. coli, spanning Met1-Val398. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8WYN0 |
| タンパク質長 | Met1-Val398 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 47.54 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | hAPG4A, Cysteine protease ATG4A, ATG4A, AUTL2, Autophagin-2, APG4A, AUT-like 2 cysteine endopeptidase, Autophagy-related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2 |
| 背景 | Cysteine protease ATG4A is a ~45 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. 1. Scherz-Shouval, R. et al. (2003) The Journal of biological chemistry 278, 14053-8. PMID: 12473658 2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837 3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651 4. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865 5. Shu, CW. et al. (2011) Journal of biomolecular screening 16, 174-82. PMID: 21245471 6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004 7. Nguyen, N. et al. (2020) The Journal of biological chemistry 295, 13584-13600. PMID: 32732290 9. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106 10. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ATG4A Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
