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| カタログ番号 | HD440012 |
|---|---|
| 説明 |
Recombinant Human ATIC Protein, N-His (HD440012) expressed in E. coli, spanning Lys225-His592. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P31939 |
| タンパク質長 | Lys225-His592 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 42.64 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR formyltransferase, AICAR transformylase, AICAR transformylase/inosine monophosphate cyclohydrolase, ATIC, Bifunctional purine biosynthesis protein ATIC, Bifunctional purine biosynthesis protein ATIC, N-terminally processed, EC:2.1.2.3, EC:3.5.4.10, IMP cyclohydrolase, IMP synthase, Inosine 5'-monophosphate cyclohydrolase, Inosinicase, PURH, Phosphoribosylaminoimidazolecarboxamide formyltransferase |
| 背景 | Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias. 1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179 2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554 3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775 4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707 5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571 |
| 注意事項 | For research use only |
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