Human AUP1 Recombinant Protein (N-His) (HV444012)

Name: Human AUP1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV444012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HV444012
Descripción	Recombinant Human AUP1 Protein, N-His (HV444012) expressed in E. coli, spanning Val201-Asp410. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9Y679
Longitud de proteína	Val201-Asp410
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	25.79 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Ancient ubiquitous protein 1, AUP1, Lipid droplet-regulating VLDL assembly factor AUP1
Fondo	Lipid droplet-regulating VLDL assembly factor AUP1 is a ~45 kDa protein. Plays a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Plays a role in lipid droplet formation. Induces lipid droplet clustering. Recruits ubiquitin-conjugating enzyme UBE2G2 to lipid droplets which facilitates its interaction with ubiquitin ligases AMFR/gp78 and RNF139/TRC8, leading to sterol-induced ubiquitination of HMGCR and its subsequent proteasomal degradation. Also required for the degradation of INSIG1, SREBF1 and SREBF2. 1. Mueller, B. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12325-30. PMID: 18711132 2. Klemm, EJ. et al. (2011) The Journal of biological chemistry 286, 37602-14. PMID: 21857022 3. Lohmann, D. et al. (2013) PloS one 8, e72453. PMID: 24039768 4. Spandl, J. et al. (2011) The Journal of biological chemistry 286, 5599-606. PMID: 21127063 5. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569 6. Zhang, J. et al. (2017) Arteriosclerosis, thrombosis, and vascular biology 37, 633-642. PMID: 28183703
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human AUP1 Protein, N-His

Referencias

Datasheet

Human AUP1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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