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| カタログ番号 | HF891012 |
|---|---|
| 説明 |
Recombinant Human BANF1 Protein, N-His (HF891012) expressed in E. coli, spanning Thr2-Leu89. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O75531 |
| タンパク質長 | Thr2-Leu89 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 12.22 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Barrier-to-autointegration factor, Breakpoint cluster region protein 1, BANF1, BCRG1, BAF |
| 背景 | Barrier-to-autointegration factor (BANF1) is a ~10 kDa protein. Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA. Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging. Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner. Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface. Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity. 1. Zheng, R. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 8997-9002. PMID: 10908652 2. Haraguchi, T. et al. (2001) Journal of cell science 114, 4575-85. PMID: 11792822 3. Segura-Totten, M. et al. (2002) The Journal of cell biology 158, 475-85. PMID: 12163470 4. Wiebe, MS. et al. (2007) Cell host & microbe 1, 187-97. PMID: 18005698 5. Kobayashi, S. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 7027-32. PMID: 25991860 6. Samwer, M. et al. (2017) Cell 170, 956-972.e23. PMID: 28841419 7. Bolderson, E. et al. (2019) Nature communications 10, 5501. PMID: 31796734 8. Guey, B. et al. (2020) Science (New York, N.Y.) 369, 823-828. PMID: 32792394 9. Lee, MS. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 1528-33. PMID: 9465049 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human BANF1 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
