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| カタログ番号 | HF756012 |
|---|---|
| 説明 |
Recombinant Human BCL10 Protein, N-His (HF756012) expressed in E. coli, spanning Met1-Gln233. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O95999 |
| タンパク質長 | Met1-Gln233 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.41 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CED-3/ICH-1 prodomain homologous E10-like regulator, CLAP, B-cell CLL/lymphoma 10, BCL10, CARD-containing molecule enhancing NF-kappa-B, Cellular homolog of vCARMEN, cCARMEN, CIPER, Bcl-10, hCLAP, Cellular-E10, CARD-like apoptotic protein, c-E10, Mammalian CARD-containing adapter molecule E10, B-cell lymphoma/leukemia 10, mE10 |
| 背景 | B-cell lymphoma/leukemia 10 (BCL10) is a ~26 kDa protein. Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation. Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex. This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity. 1. Koseki, T. et al. (1999) The Journal of biological chemistry 274, 9955-61. PMID: 10187770 2. Srinivasula, SM. et al. (1999) The Journal of biological chemistry 274, 17946-54. PMID: 10364242 3. Costanzo, A. et al. (1999) The Journal of biological chemistry 274, 20127-32. PMID: 10400625 4. Qiao, Q. et al. (2013) Molecular cell 51, 766-79. PMID: 24074955 5. Torres, JM. et al. (2014) The Journal of clinical investigation 124, 5239-48. PMID: 25365219 6. Wu, CJ. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3023-8. PMID: 18287044 7. Yang, YK. et al. (2016) The Journal of biological chemistry 291, 25921-25936. PMID: 27777308 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human BCL10 Protein, N-His
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