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| カタログ番号 | HP138012 |
|---|---|
| 説明 |
Recombinant Human FBXW7 Protein, N-His (HP138012) expressed in E. coli, spanning Asp279-Val515. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q969H0,F1MNN4 |
| タンパク質長 | Asp279-Val515 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human), Bos taurus (Bovine) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW7, FBX30, FBXW7, SEL-10, SEL10, hAgo, hCdc4 |
| 背景 | F-box/WD repeat-containing protein 7 (FBXW7) is a ~79 kDa protein. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter brings them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NFE2L1, NOTCH2, MCL1, MLST8, RICTOR, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. Involved in bone homeostasis and negative regulation of osteoclast differentiation. 1. Hao, B. et al. (2007) Molecular cell 26, 131-43. PMID: 17434132 2. Duda, DM. et al. (2012) Molecular cell 47, 371-82. PMID: 22748924 3. Gorelik, M. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 3527-32. PMID: 26976582 4. Yalla, K. et al. (2018) Molecular psychiatry 23, 1278-1286. PMID: 28727686 5. Zhang, E. et al. (2022) Cancer science 113, 91-108. PMID: 34741373 6. Stephenson, SEM. et al. (2022) American journal of human genetics 109, 601-617. PMID: 35395208 7. Zhang, Q. et al. (2016) Molecular cell 61, 419-433. PMID: 26774286 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human FBXW7 Protein, N-His
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