Human CA4 Recombinant Protein (N-His) (HB731012)

Name: Human CA4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB731012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB731012
Description	Recombinant Human CA4 Protein, N-His (HB731012) expressed in E. coli, spanning Ala19-Lys283. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P22748
Longueur de protéine	Ala19-Lys283
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	32.53 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Carbonic anhydrase 4, Carbonate dehydratase IV, CA-IV, CA4, Carbonic anhydrase IV
Contexte	Carbonic anhydrase 4 (CA4) is a ~35 kDa protein. Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. 1. Yang, Z. et al. (2005) Human molecular genetics 14, 255-65. PMID: 15563508 2. Temperini, C. et al. (2006) Journal of medicinal chemistry 49, 3019-27. PMID: 16686544 3. Temperini, C. et al. (2006) Chemistry (Weinheim an der Bergstrasse, Germany) 12, 7057-66. PMID: 16807956 4. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 628-35. PMID: 17127057 5. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 2210-5. PMID: 17314045 6. Alvarez, BV. et al. (2007) Investigative ophthalmology & visual science 48, 3459-68. PMID: 17652713 7. Köhler, K. et al. (2007) Angewandte Chemie (International ed. in English) 46, 7697-9. PMID: 17705204 8. Di Fiore, A. et al. (2009) Proteins 74, 164-75. PMID: 18618712 9. Crocetti, L. et al. (2009) Bioorganic & medicinal chemistry letters 19, 1371-5. PMID: 19186056 10. Maresca, A. et al. (2009) Journal of the American Chemical Society 131, 3057-62. PMID: 19206230
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CA4 Protein, N-His

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Human CA4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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