ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
32.31 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
hCARD9, CARD9, Caspase recruitment domain-containing protein 9
Contexte
Caspase recruitment domain-containing protein 9 (CARD9) is a ~62 kDa protein. Adapter protein that plays a key role in innate immune response against fungi by forming signaling complexes downstream of C-type lectin receptors. CARD9-mediated signals are essential for antifungal immunity against a subset of fungi from the phylum Ascomycota. Transduces signals in myeloid cells downstream of C-type lectin receptors CLEC7A (dectin-1), CLEC6A (dectin-2) and CLEC4E (Mincle), which detect pathogen-associated molecular pattern metabolites (PAMPs), such as fungal carbohydrates, and trigger CARD9 activation. Upon activation, CARD9 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. CARD9 signaling in antigen-presenting cells links innate sensing of fungi to the activation of adaptive immunity and provides a cytokine milieu that induces the development and subsequent of interleukin 17-producing T helper (Th17) cells.
1. Alves de Medeiros, AK. et al. (2016) Journal of clinical immunology 36, 204-9. PMID: 26961233
2. Imanaka, Y. et al. (2021) Journal of clinical immunology 41, 975-986. PMID: 33558980
3. Wang, X. et al. (2014) The Journal of allergy and clinical immunology 133, 905-8.e3. PMID: 24231284
4. Lanternier, F. et al. (2015) The Journal of infectious diseases 211, 1241-50. PMID: 25057046
5. Lanternier, F. et al. (2015) The Journal of allergy and clinical immunology 135, 1558-68.e2. PMID: 25702837
6. Gavino, C. et al. (2016) The Journal of allergy and clinical immunology 137, 1178-1188.e7. PMID: 26521038
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