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| カタログ番号 | HB991012 |
|---|---|
| 説明 |
Recombinant Human CASP1/Caspase-1 Protein, N-His (HB991012) expressed in E. coli, spanning Asn120-Asp297. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P29466 |
| タンパク質長 | Asn120-Asp297 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.14 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | IL1BC, Interleukin-1 beta-converting enzyme, p45, Caspase-1, IL-1BC, IL-1 beta-converting enzyme, CASP1, IL1BCE, ICE, CASP-1, Interleukin-1 beta convertase |
| 背景 | Caspase-1 (CASP1) is a ~45 kDa protein. Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. 1. Gaggero, A. et al. (2004) Oncogene 23, 7552-60. PMID: 15326478 2. Feng, Q. et al. (2004) Genomics 84, 587-91. PMID: 15498465 3. Thornberry, NA. et al. (1992) Nature 356, 768-74. PMID: 1574116 4. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003 5. Ball, DP. et al. (2020) Life science alliance 3. PMID: 32051255 6. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 7. Alnemri, ES. et al. (1995) The Journal of biological chemistry 270, 4312-7. PMID: 7876192 8. Akita, K. et al. (1997) The Journal of biological chemistry 272, 26595-603. PMID: 9334240 9. Wang, K. et al. (2020) Cell 180, 941-955.e20. PMID: 32109412 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CASP1/Caspase-1 Protein, N-His
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