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| カタログ番号 | HX967012 |
|---|---|
| 説明 |
Recombinant Human CASP7 Protein, N-His (HX967012) expressed in E. coli, spanning Ala24-Asp198. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P55210 |
| タンパク質長 | Ala24-Asp198 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.01 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CASP7, ICE-LAP3, CASP-7, MCH3, Caspase-7, CMH-1, ICE-like apoptotic protease 3, Apoptotic protease Mch-3 |
| 背景 | Caspase-7 (CASP7) is a ~34 kDa protein. Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins. Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences. Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7. Acts as an effector caspase involved in the execution phase of apoptosis: following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1. Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins. 1. Chai, J. et al. (2001) Cell 104, 769-80. PMID: 11257230 2. Huang, Y. et al. (2001) Cell 104, 781-90. PMID: 11257231 3. Chai, J. et al. (2001) Cell 107, 399-407. PMID: 11701129 4. Hardy, JA. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 12461-6. PMID: 15314233 5. Denault, JB. et al. (2006) Molecular cell 23, 523-33. PMID: 16916640 6. Young, JE. et al. (2007) The Journal of biological chemistry 282, 30150-60. PMID: 17646170 7. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680 8. Hardy, JA. et al. (2009) The Journal of biological chemistry 284, 26063-9. PMID: 19581639 9. Fernandes-Alnemri, T. et al. (1995) Cancer research 55, 6045-52. PMID: 8521391 10. Lippke, JA. et al. (1996) The Journal of biological chemistry 271, 1825-8. PMID: 8567622 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CASP7 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
