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| カタログ番号 | HB638012 |
|---|---|
| 説明 |
Recombinant Human CBL Protein, N-GST (HB638012) expressed in E. coli, spanning Ser558-Gly816. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P22681 |
| タンパク質長 | Ser558-Gly816 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 54.66 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CBL, RING finger protein 55, Proto-oncogene c-Cbl, CBL2, E3 ubiquitin-protein ligase CBL, RING-type E3 ubiquitin transferase CBL, Casitas B-lineage lymphoma proto-oncogene, Signal transduction protein CBL, RNF55 |
| 背景 | E3 ubiquitin-protein ligase CBL is a ~99 kDa protein. E3 ubiquitin-protein ligase that acts as a negative regulator of many signaling pathways by mediating ubiquitination of cell surface receptors. Accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and mediates their ubiquitination to terminate signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Ubiquitinates EGFR and SPRY2. 1. Joazeiro, CA. et al. (1999) Science (New York, N.Y.) 286, 309-12. PMID: 10514377 2. Howlett, CJ. et al. (2002) Oncogene 21, 1707-16. PMID: 11896602 3. Kim, M. et al. (2004) Oncogene 23, 1645-55. PMID: 14661060 4. Miyazaki, T. et al. (2004) The Journal of biological chemistry 279, 17660-6. PMID: 14739300 5. Kaabeche, K. et al. (2004) The Journal of biological chemistry 279, 36259-67. PMID: 15190072 6. Bonaventure, J. et al. (2007) The FEBS journal 274, 3078-93. PMID: 17509076 7. Dufour, C. et al. (2008) Bone 42, 1032-9. PMID: 18374639 8. Wehrle, C. et al. (2009) The Biochemical journal 423, 375-80. PMID: 19689429 9. Sévère, N. et al. (2011) The Journal of biological chemistry 286, 24443-50. PMID: 21596750 10. Deschamps, T. et al. (2017) Journal of virology 91. PMID: 28381567 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CBL Protein, N-GST
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