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Обзор
Каталожный номерHD272012
Описание
Recombinant Human CCDC88A Protein, N-His (HD272012) expressed in E. coli, spanning Met1-Phe223. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ3V6T2
Длина белкаMet1-Phe223
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 27.85 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияAPE, Akt phosphorylation enhancer, CCDC88A, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, GRDN, Girders of actin filament, Girdin, HkRP1, Hook-related protein 1, KIAA1212
Фон

Girdin is a ~216 kDa protein. Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration.

1. Garcia-Marcos, M. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 3178-83. PMID: 19211784
2. Gupta, V. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E5721-30. PMID: 27621449
3. Lin, C. et al. (2011) Science signaling 4, ra64. PMID: 21954290
4. López-Sánchez, I. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 5510-5. PMID: 23509302
5. Lin, C. et al. (2014) Molecular biology of the cell 25, 3654-71. PMID: 25187647
6. Enomoto, A. et al. (2005) Developmental cell 9, 389-402. PMID: 16139227
7. Ghosh, P. et al. (2010) Molecular biology of the cell 21, 2338-54. PMID: 20462955
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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