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| カタログ番号 | HX131012 |
|---|---|
| 説明 |
Recombinant Human CCL20/MIP-3-alpha Protein, N-His (HX131012) expressed in E. coli, spanning Ser28-Met96. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P78556 |
| タンパク質長 | Ser28-Met96 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 10.25 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Beta-chemokine exodus-1, SCYA20, LARC, C-C motif chemokine 20, Liver and activation-regulated chemokine, Small-inducible cytokine A20, MIP-3-alpha, CCL20, MIP3A, Macrophage inflammatory protein 3 alpha, CC chemokine LARC |
| 背景 | C-C motif chemokine 20 (CCL20/MIP-3-alpha) is a ~10 kDa protein. Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. 1. Schutyser, E. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 165, 4470-7. PMID: 11035086 2. Nelson, RT. et al. (2001) Genomics 73, 28-37. PMID: 11352563 3. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036 4. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174 5. Hieshima, K. et al. (1997) The Journal of biological chemistry 272, 5846-53. PMID: 9038201 6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988 7. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636 8. Hromas, R. et al. (1997) Blood 89, 3315-22. PMID: 9129037 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CCL20/MIP-3-alpha Protein, N-His
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