Human CCNT1 Recombinant Protein (N-His) (HC948012)

Name: Human CCNT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC948012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор
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Даташит
SDS

Обзор

Каталожный номер	HC948012
Описание	Recombinant Human CCNT1 Protein, N-His (HC948012) expressed in E. coli, spanning Met1-Ser290. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O60563
Длина белка	Met1-Ser290
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	35.52 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	CCNT1, CycT1, Cyclin-T, Cyclin-T1
Фон	Cyclin-T1 is a ~80 kDa protein. Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II. 1. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376 2. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377 3. Ghosh, K. et al. (2018) Cell reports 24, 2141-2154.e6. PMID: 30134174 4. Fu, H. et al. (2022) Nature cell biology 24, 513-525. PMID: 35393539 5. Lu, H. et al. (2018) Nature 558, 318-323. PMID: 29849146
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Human CCNT1 Recombinant Protein (N-His)

Ссылки

Даташит

Human CCNT1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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