Human CCT4 Recombinant Protein (N-His) (HW560012)

Name: Human CCT4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW560012
Price: 211 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue	HW560012
Description	Recombinant Human CCT4 Protein, N-His (HW560012) expressed in E. coli, spanning Gln25-Arg539. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P50991
Longueur de protéine	Gln25-Arg539
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	57.99 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CCTD, T-complex protein 1 subunit delta, TCP-1-delta, Stimulator of TAR RNA-binding, CCT4, CCT-delta, SRB
Contexte	T-complex protein 1 subunit delta (CCT4) is a ~57 kDa protein. Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. 1. Freund, A. et al. (2014) Cell 159, 1389-403. PMID: 25467444 2. Gestaut, D. et al. (2022) Cell 185, 4770-4787.e20. PMID: 36493755 3. Kelly, JJ. et al. (2022) Nature structural & molecular biology 29, 420-429. PMID: 35449234 4. Liu, C. et al. (2023) Communications biology 6, 531. PMID: 37193829 5. Seo, S. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 1488-93. PMID: 20080638
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CCT4 Protein, N-His

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Human CCT4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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