Human B7-1/CD80 Recombinant Protein (C-Fc) (HW630021)

Name: Human B7-1/CD80 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: HW630021
Price: 298 USD
Availability: InStock

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100ug
1mg

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SDS

Aperçu

Numéro de catalogue	HW630021
Description	Recombinant Human CD80/B7-1 Protein, C-Fc (HW630021) expressed in Mammalian Cells, spanning Met1-Asn242. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	Mammalian cells
Numéro d'accès	P33681
Longueur de protéine	Met1-Asn242
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	54.00 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Activation B7-1 antigen, B7, BB1, CD28LG, CD28LG1, CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Contexte	T-lymphocyte activation antigen CD80 is a ~33 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. 1. Lu, B. et al. (2024) Acta pharmacologica Sinica 45, 1214-1223. PMID: 38467718 2. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 3. Boulougouris, G. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1809-16. PMID: 10438913 4. Vandenborre, K. et al. (1999) Immunology 98, 413-21. PMID: 10583602 5. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502 6. Frauwirth, KA. et al. (2002) Immunity 16, 769-77. PMID: 12121659 7. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298
Note	For research use only

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Bioactivity

Immobilized HW630021 Human CD80/B7-1 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB651011 Human CD152/CTLA4 Recombinant Protein (C-His), the EC 50 is 250 ng/mL.
Bioactivity

Immobilized HW630021 Human CD80/B7-1 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HB651021 Human CD152/CTLA4 Recombinant Protein (C-Fc), the EC 50 is 143 ng/mL.