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Human CERS6 Recombinant Protein (N-His) (HD887012)

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Aperçu
Numéro de catalogueHD887012
Description
Recombinant Human CERS6 Protein, N-His (HD887012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ6ZMG9
Longueur de protéineGlu56-Asp177
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsCERS6, CerS6, Ceramide synthase 6, EC:2.3.1.-, EC:2.3.1.291, LAG1 longevity assurance homolog 6, LASS6, Sphingoid base N-palmitoyltransferase CERS6
Contexte

Ceramide synthase 6 is a ~44 kDa protein. Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA). Can use other acyl donors, but with less efficiency. N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Ceramides generated by CERS6 play a role in inflammatory response. Acts as a regulator of metabolism and hepatic lipid accumulation.

1. Mesika, A. et al. (2007) The Journal of biological chemistry 282, 27366-27373. PMID: 17609214
2. Lahiri, S. et al. (2007) FEBS letters 581, 5289-94. PMID: 17977534
3. Russo, SB. et al. (2013) The Journal of biological chemistry 288, 13397-409. PMID: 23530041
4. Sassa, T. et al. (2016) The Journal of biological chemistry 291, 7477-87. PMID: 26887952
5. Jojima, K. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 3318-3335. PMID: 31916624
6. Pascoa, TC. et al. (2025) Nature structural & molecular biology 32, 431-440. PMID: 39528795
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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