Human CHMP2A Recombinant Protein (N-His) (HT433012)

Name: Human CHMP2A Recombinant Protein (N-His)
Brand: abinScience
SKU: HT433012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HT433012
Description	Recombinant Human CHMP2A Protein, N-His (HT433012) expressed in E. coli, spanning Pro11-Asn181. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O43633
Longueur de protéine	Pro11-Asn181
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	22.10 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Vps2-1, Putative breast adenocarcinoma marker BC-2, CHMP2A, hVps2-1, CHMP2a, Vacuolar protein sorting-associated protein 2-1, Charged multivesicular body protein 2a, CHMP2, BC2, Chromatin-modifying protein 2a
Contexte	Charged multivesicular body protein 2a (CHMP2A) is a ~25 kDa protein. Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. 1. Guizetti, J. et al. (2011) Science (New York, N.Y.) 331, 1616-20. PMID: 21310966 2. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712 3. Gu, M. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2166-E2175. PMID: 28242692
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CHMP2A Protein, N-His

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Human CHMP2A Recombinant Protein (N-His).pdf

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