Human CLCN2 Recombinant Protein (N-His) (HA683012)

Name: Human CLCN2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA683012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HA683012
Descripción	Recombinant Human CLCN2 Protein, N-His (HA683012) expressed in E. coli, spanning Leu568-Gly847. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P51788
Longitud de proteína	Leu568-Gly847
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	33.17 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Chloride channel protein 2; ClC-2; CLCN2
Fondo	Chloride channel protein 2 is a ~98 kDa protein. Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity. Contributes to chloride conductance involved in neuron excitability. 1. Peña-Münzenmayer, G. et al. (2005) Journal of cell science 118, 4243-52. PMID: 16155254 2. Blanz, J. et al. (2007) The Journal of neuroscience : the official journal of the Society for Neuroscience 27, 6581-9. PMID: 17567819 3. Saint-Martin, C. et al. (2009) Human mutation 30, 397-405. PMID: 19191339 4. Stölting, G. et al. (2013) Pflugers Archiv : European journal of physiology 465, 1423-37. PMID: 23632988 5. Scholl, UI. et al. (2018) Nature genetics 50, 349-354. PMID: 29403011 6. Fernandes-Rosa, FL. et al. (2018) Nature genetics 50, 355-361. PMID: 29403012 7. Xu, P. et al. (2023) Human genetics 142, 577-593. PMID: 36964785 8. Xu, M. et al. (2024) eLife 12. PMID: 38345841 9. Jeworutzki, E. et al. (2012) Neuron 73, 951-61. PMID: 22405205
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human CLCN2 Protein, N-His

Referencias

Datasheet

Human CLCN2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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