Human CLCN2 Recombinant Protein (N-His) (HA683012)

Name: Human CLCN2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA683012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HA683012
Описание	Recombinant Human CLCN2 Protein, N-His (HA683012) expressed in E. coli, spanning Leu568-Gly847. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P51788
Длина белка	Leu568-Gly847
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	33.17 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Chloride channel protein 2; ClC-2; CLCN2
Фон	Chloride channel protein 2 is a ~98 kDa protein. Voltage-gated and osmosensitive chloride channel. Forms a homodimeric channel where each subunit has its own ion conduction pathway. Conducts double-barreled currents controlled by two types of gates, two fast glutamate gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Displays inward rectification currents activated upon membrane hyperpolarization and extracellular hypotonicity. Contributes to chloride conductance involved in neuron excitability. 1. Peña-Münzenmayer, G. et al. (2005) Journal of cell science 118, 4243-52. PMID: 16155254 2. Blanz, J. et al. (2007) The Journal of neuroscience : the official journal of the Society for Neuroscience 27, 6581-9. PMID: 17567819 3. Saint-Martin, C. et al. (2009) Human mutation 30, 397-405. PMID: 19191339 4. Stölting, G. et al. (2013) Pflugers Archiv : European journal of physiology 465, 1423-37. PMID: 23632988 5. Scholl, UI. et al. (2018) Nature genetics 50, 349-354. PMID: 29403011 6. Fernandes-Rosa, FL. et al. (2018) Nature genetics 50, 355-361. PMID: 29403012 7. Xu, P. et al. (2023) Human genetics 142, 577-593. PMID: 36964785 8. Xu, M. et al. (2024) eLife 12. PMID: 38345841 9. Jeworutzki, E. et al. (2012) Neuron 73, 951-61. PMID: 22405205
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human CLCN2 Protein, N-His

Ссылки

Даташит

Human CLCN2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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