Human CLCNKA Recombinant Protein (N-His) (HA681012)

Name: Human CLCNKA Recombinant Protein (N-His)
Brand: abinScience
SKU: HA681012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HA681012
Descripción	Recombinant Human CLCNKA Protein, N-His (HA681012) expressed in E. coli, spanning Ala507-Lys687. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P51800
Longitud de proteína	Ala507-Lys687
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	22.16 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Chloride channel protein ClC-Ka; Chloride channel Ka; ClC-K1; CLCNKA
Fondo	Chloride channel protein ClC-Ka is a ~75 kDa protein. Anion-selective channel permeable to small monovalent anions with ion selectivity for chloride > bromide > nitrate > iodide. Forms a homodimeric channel where each subunit has its own ion conduction pathway. May conduct double-barreled currents controlled by two types of gates, two fast gates that control each subunit independently and a slow common gate that opens and shuts off both subunits simultaneously. Assembles with the regulatory subunit BSND/Barttin for sorting at the basolateral plasma membrane domain and functional switch to the ion conducting state. CLCNKA:BSND channels display mostly a linear current-voltage relationship with fast gating at negative potentials. 1. Estévez, R. et al. (2001) Nature 414, 558-61. PMID: 11734858 2. Waldegger, S. et al. (2002) Pflugers Archiv : European journal of physiology 444, 411-8. PMID: 12111250 3. Nozu, K. et al. (2008) Journal of medical genetics 45, 182-6. PMID: 18310267 4. Janssen, AG. et al. (2009) Journal of the American Society of Nephrology : JASN 20, 145-53. PMID: 18776122 5. Riazuddin, S. et al. (2009) American journal of human genetics 85, 273-80. PMID: 19646679 6. Fischer, M. et al. (2010) Journal of the American Society of Nephrology : JASN 21, 1281-9. PMID: 20538786
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human CLCNKA Protein, N-His

Referencias

Datasheet

Human CLCNKA Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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